P01

To understand how the complex architecture of the ER is generated, we propose to systematically analyze the major ER membrane-shaping proteins of the Reticulon and REEP families. We will first characterize their molecular environments, second determine their precise localization in cells and third investigate their functions by a targeted degradation approach. This work will help to elucidate how different ER subdomains are established and enable the various functions of the organelle.